細(xì)胞外酸化速率ECAR探針
細(xì)胞外ECAR納米探針是一種被充分證明了不會(huì)穿透細(xì)胞膜進(jìn)入細(xì)胞的納米探針，探針具有優(yōu)異的水中分散性和生物相容性，能在不影響細(xì)胞正常生理代謝的情況下，通過(guò)磷光壽命測(cè)量實(shí)現(xiàn)細(xì)胞外的pH值或酸化程度的實(shí)時(shí)測(cè)量。探針在不同的pH時(shí)可呈現(xiàn)出不同的顏色，用戶可以簡(jiǎn)便地通過(guò)顏色變化來(lái)判斷細(xì)胞培養(yǎng)基的pH變化。探針的磷光壽命隨著pH變化非常明顯。探針長(zhǎng)達(dá)毫秒ms級(jí)的磷光壽命，使得測(cè)量的準(zhǔn)確度和分辨率大幅度提高，試劑的測(cè)量精度高達(dá)0.005 pH變化，非常適用于細(xì)胞外環(huán)境微小pH變化的測(cè)量。
該試劑具有優(yōu)異的穩(wěn)定性，在37度細(xì)胞培養(yǎng)環(huán)境中，其磷光壽命在連續(xù)測(cè)量180分鐘時(shí)，均未出現(xiàn)明顯的波動(dòng)，表明試劑具有良好的測(cè)量穩(wěn)定性和可靠性。通過(guò)連續(xù)不斷地測(cè)量細(xì)胞外pH的變化，可以通過(guò)pH變化速率計(jì)算出細(xì)胞外酸化速率(ExtraCellular Acidificatioin Rate, ECAR)值，實(shí)現(xiàn)細(xì)胞能量代謝水平，尤其是經(jīng)過(guò)糖酵解途徑的能量代謝水平的定量評(píng)估。
該試劑可以在96孔板中實(shí)現(xiàn)活細(xì)胞ECAR水平的測(cè)量，無(wú)需采購(gòu)昂貴的Seahorse或者O2K設(shè)備和耗材進(jìn)行測(cè)量，客戶只需配備帶有熒光檢測(cè)功能、最好是時(shí)間分辨熒光(time-resolved fluorescence)功能的酶標(biāo)儀，就可以實(shí)現(xiàn)ECAR的高通量測(cè)量，大大節(jié)約了測(cè)量成本。
The extracellular acidification rate (ECAR) nanoprobe is a nanomaterial that has been thoroughly proven not to penetrate cell membranes. It exhibits excellent water dispersibility and biocompatibility, allowing for real-time measurement of extracellular pH or acidification levels via phosphorescence lifetime measurements, the nanoprobe do not interfere normal cellular physiological metabolism. The nanoprobe exhibits different colors at different pH values, enabling users to easily distinguish pH changes in the culture media through color variations. The phosphorescence lifetime of the nanoprobe varies significantly with pH changes. The nanoprobe's long phosphorescence lifetime, (upto millisecond level), significantly improves measurement accuracy and resolution. The reagent has a measurement precision as high as 0.005 pH units, making it highly suitable for measuring subtle pH changes in the extracellular environment.
This reagent exhibits excellent photostability. In a 37°C cell culture environment, its phosphorescence lifetime remains remarkably stable over continuous measurements for 180 minutes, indicating excellent measurement stability and reliability. By continuously measuring extracellular pH changes, the extracellular acidification rate (ECAR) can be calculated based on the rate of pH change, enabling quantitative evaluation of cellular energy metabolism levels, especially those involving the glycolytic pathway.
This reagent can be used to measure the ECAR levels of live cells in 96-well plates. There is no need to purchase expensive Seahorse or O2K equipment and consumables. Customers only need a microplate reader equipped with fluorescence detection capabilities, preferably time-resolved fluorescence, to achieve high-throughput ECAR measurements, significantly reducing measurement costs.
富途生物研發(fā)的細(xì)胞外酸化速率ECAR試劑具有非常大的Stokes位移（~280nm），可有效避免生物自發(fā)熒光的干擾；試劑具有長(zhǎng)達(dá)毫秒級(jí)的磷光壽命，可簡(jiǎn)單地通過(guò)時(shí)間分辨熒光測(cè)量技術(shù)進(jìn)一步提高抗生物自發(fā)熒光干擾的能力，并大幅度提高測(cè)量的準(zhǔn)確度和穩(wěn)定性，從而實(shí)現(xiàn)長(zhǎng)達(dá)數(shù)小時(shí)的細(xì)胞外酸化速率的測(cè)量，是測(cè)量細(xì)胞糖酵解反應(yīng)和細(xì)胞外酸化速率的重要測(cè)量工具。
The extracellular acidification rate (ECAR) reagent developed by Fluorotool Biotech has an exceptionally large Stokes shift (~280 nm), effectively minimizing interference from endogenous bioluminescence. With a phosphorescent lifetime extending to the millisecond range, the reagent can be further enhanced for bioluminescence resistance through simple time-resolved fluorescence measurement techniques. This significantly improves measurement accuracy and stability, enabling continuous ECAR monitoring for several hours. It is a crucial tool for measuring cellular glycolytic reactions and extracellular acidification rates.