細胞外OCR探針
超小尺寸納米探針，具有優(yōu)異的生物相容性和在水中的穩(wěn)定性，在4℃水溶液中避光保存1年以上，沒有發(fā)現(xiàn)明顯的沉降和團聚，產(chǎn)品在出廠前已進行滅菌處理，可直接使用。細胞外OCR試劑具有優(yōu)異的氧氣響應(yīng)靈敏度（中圖），可通過熒光強度和門控熒光壽命測量技術(shù)實現(xiàn)細胞外或培養(yǎng)基中氧濃度的連續(xù)不間斷測量，是進行細胞OCR測量的良好工具。如右圖所示，細胞外OCR試劑可根據(jù)客戶需求，定制不同波長、不同熒光壽命的試劑，避免與您細胞成像的染料之間發(fā)生光譜重疊，為您提供定制化的解決方案。
We had developed ultra-small nanoprobe for measuring extracellular oxygen consumption rate (OCR). The reagent has excellent biocompatibility and stability in water. It can be stored in a solution at 4 ℃ for more than 1 year without obvious sedimentation or aggregation. The product has been sterilized before delivery and can be used directly without any treatment. Extracellular OCR reagents have excellent oxygen response sensitivity (as shown in the middle figure), and can achieve continuous measurement of oxygen concentration outside cells or in culture media via fluorescence intensity and time-gated fluorescence lifetime measurement techniques. They are an ideal tool for extracellular OCR measurement. As shown in the right figure, extracellular OCR reagents can be customized with different wavelengths and fluorescence lifetimes, we also offer customized solution according to your special needs in order to avoid spectral overlap with the other cell-staining dyes.
細胞外OCR試劑已經(jīng)被證明了不會被細胞內(nèi)吞，可以穩(wěn)定地懸浮在培養(yǎng)基中。通過連續(xù)測量胞外的氧氣消耗速率，獲得活細胞的線粒體功能狀態(tài)，如左圖所示，通過在培養(yǎng)基中添加藥物調(diào)控活細胞線粒體功能，可有效地調(diào)控細胞的耗氧速率OCR，從而實現(xiàn)無創(chuàng)、不影響細胞正常能量代謝的細胞OCR分析，該試劑測量速度快、準確度高、測量通量大，是進行活細胞線粒體功能評估和能量代謝分析的有力工具。在電穿孔Electroporation技術(shù)的幫助下，該試劑可快速擴散到細胞液中，實現(xiàn)細胞質(zhì)中的氧濃度實時測量。
The extracellular oxygen consumption rate (OCR) reagent has been proven not to be endocytosed by living cells and can be stably suspended in the culture medium for long duration. By continuously measuring the oxygen consumption rate outside the cell, the mitochondrial function state of live cells can be obtained, as shown in the left figure. By regulating the mitochondrial function of live cells in the culture medium by adding mitochondrial inhibitors, the oxygen consumption rate (OCR) of cells can be effectively regulated, thereby achieving non-invasive and non-disruptive cell OCR analysis without affecting the normal energy metabolism of cells. The reagent has a fast response, high accuracy, and high measurement throughput, and is a powerful tool for live cell mitochondrial function assessment and energy metabolism analysis. With the help of Electroporation technology, the reagent can quickly diffuse into the cell fluid and realize real-time measurement of oxygen concentration in the cytoplasm.
細胞外OCR試劑不僅適用于高通量的96孔板進行細胞、藥物和培養(yǎng)條件的篩選，也能通過電穿孔Electroporation技術(shù)導(dǎo)入到細胞液中，進行細胞液中的顯微成像，實現(xiàn)單/活細胞細胞液中氧濃度的測量，對類器官、組織切片也有很好的適用性。這種試劑還可以被噴灑到植物葉片上，進行植物光合作用的分析和研究。
The extracellular OCR reagent is not only suitable for high-throughput 96-well plate screening of cells, drugs, and culture conditions, but can also be useful in studying oxygen concentration in cytoplasm via electroporation. The reagent enables the measurement of oxygen concentration in single/live cells in the extracellular space, in cytoplasm, and is also suitable for organoids, tissue sections. This reagent can also be sprayed onto plant leaves for analysis and research on plant photosynthesis.